Rabbit IgG ELISA Kit

Product overview:

Cytodiagnostics Rabbit IgG ELISA kit is a sensitive and accurate assay for the quantitative determination of Rabbit IgG levels in serum, plasma and cell culture supernatants.

The Rabbit IgG ELISA kit is based on the antibody sandwich principle. A microtiter plate coated with a capture antibody specific to Rabbit IgG Fc has been blocked and stabilized to create the solid phase of the assay. To perform the assay, samples, standards, and controls are added directly to the wells of the plate. After washing away unbound IgG, an HRP-conjugated Detection Antibody Solution is added and binds to the heavy and light chains of the captured Rabbit IgG protein that were immobilized by the capture antibody, completing the sandwich. The wells are washed and a tetramethylbenzidine (TMB) Substrate Solution is added. A blue colour develops in proportion to the amount of bound Rabbit IgG. The color development is stopped using Stop Solution, which turns the blue end-product to yellow and the optical density (OD) of the yellow product is measured at 450 nm on a microtiter plate reader.

Our ELISA kit has several benefits:

  • Ready to use. Simply add standards and samples directly to wells. No need for lengthy blocking or rehydration steps.
  • High sensitivity, specificity and reproducibility.
  • Fully validated in the sample types listed below.
  • 96-well plate is breakable into 12 x 8 well strips.

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Product Specification

Protein name

Rabbit IgG

Species reactivity

Rabbit

Assay format

Solid-phase Sandwich ELISA (quantitative)

Sample type

Serum, Plasma, Cell culture supernatant

Sample volume

100 μL

Assay length

3.5 hrs

Analytical sensitivity

<20 pg/mL

Assay range

31.25 – 2000 pg/mL

Intra-assay CV%

<8%

Inter-assay CV%

<14%

Recovery%

95.3% (Serum)

91.6% (Plasma)

88.9% (Culture media)

Detection & Instrument

Colorimetric, Microplate Reader