Supplied with Kit
- 70nm standard Gold Nanourchins (100ml, conc. OD1)
- 12 conjugation optimization buffers (1.5ml each)
- 10% NaCl (100ml)
- Protein resuspension buffer
- Detailed conjugation optimization protocol
Kit contains enough material to optimize 10 protein conjugates, or one optimization reaction and scale-up production of 20ml of conjugate at a final optical density of OD3.
Gold Nanourchin conjugates have been used for a wide range of biological applications including the use as probes in light and electron microscopy (TEM) and for membrane blotting (immunogold dot-blot); especially where large surface plasmon resonance shifts are required.
Stable Gold Nanourchin conjugates can readily be prepared by passive adsorption of a protein to the gold nanoparticle surface, which is mediated by electrostatic and hydrophobic interactions between the protein and the surface layer of the colloidal gold.
Our Gold Nanourchin protein conjugation optimization kits are supplied as complete and easy to use systems for increased success rates in the development of good quality gold nanoparticle conjugates.