MWCO Ultrafiltration Spin Columns, 100kDa

"sometimes you just need a couple columns"

Product Description

Sample cleanup, desalting, concentrating centrifugal filters. Simple one-spin function for rapid concentration of samples, and removal of small contaminants (salt, dyes, radioactive labels). Polyethersulfone (PES) membrane for maximum recovery of protein and DNA.

Ultrafiltration spin units offer a simple, one-step procedure for desalting and concentration of proteins and other macromolecules with greater than 90% recovery. Utilizing a molecular weight cut-off polyethersulfone (PES) filter membrane, the spin device is able to retain macromolecules having a greater MW, and filtrate salts and other molecules of smaller MW. Because of low protein/DNA binding properties of the PES filter, loss of the retentate on the filter is minimized. The retentate can be recovered at excellent rate.

Four molecular weight cut-off (MWCO) sizes are provided to cover a broad range of molecular weights: 3 kDa, 10 kDa, 30 kDa, and 100 kDa. It is recommended to choose a MWCO 1/2 to 1/3 smaller than the protein to be concentrated. For instance, to concentrate a protein of 80 kDa, we recommend to start with the 30 kDa MWCO spin unit.

Product Features

• Simple, rapid spin for simultaneous desalting and concentration (up to 75-fold concentration);
  
• Buffer exchange in two quick steps;
• Faster filtration - high flux PES membrane is heat-sealed to the housing unit;
  
• High recovery of the retentate - the PES membrane has very low adsorption properties for protein and DNA/RNA and the filter housing unit is also constructed with low-binding materials;
  
• Feasibility of collecting the filtrate - it is flexible to choose either the retentate or the filtrate to keep;
  
• Optimized designs for better performance:

1. Built-in dead stop pocket at the filter unit bottom - no risk of drying out during spin;
   
2. Vertical position of the filter membrane - less clogging/fouling issues;
   

• Less processing time, less buffers, and less mess compared to dialysis;
  
• Flexible throughput - it is easy to perform multiple samples in parallel or to scale up with the same sample;
• Free of detectable DNase/RNase contamination.

Applications:

• Concentration, desalting of proteins, enzymes, antibodies, DNA/RNA and other macromolecules

• Rapid removal of small contaminants (salts, dyes, detergent, and additives)

• Post-modification removal of labeled amino acids and nucleotides, or other unincorporated labels
  

• Sample pre-treatment prior to HPLC

• Deproteinization

• Recovery of biomolecules from cell culture supernatants and cell lysates

• Concentrating virus from cell culture supernatants