Silver Nanoparticle Product Selection Guide

Choose a Silver Nanoparticle Product Based on Your Particular Application

 Application Silver Nanoparticle Size Range Surface Chemistry Benefits
Protein Conjugation 10nm-100nm Standard (citrate) Quick
NHS Covalent conjugation to primary amines, increased conjugate stability, less non-specific protein binding.
Carboxyl
Covalent conjugation to primary amines, increased conjugate stability, less non-specific protein binding.
Streptavidin Conjugation to biotinylated ligands.
Modification with thiolated ligands 10nm-100nm Standard (citrate-coated) Classic starting material, no additional stabilizers added.
Oligonucleotide Conjugation 10nm-20nm Standard (citrate) Ideal for conjugation of thiol-modified oligos to small particle sizes (10nm-20nm). Does not work well for larger particles.
10nm-100nm NHS Ideal for covalent conjugation of amine-modified oligos. Final conjugate will have a PEG-linker between oligo and silver surface.
Immunoblotting/Western Blot 10nm-30nm Secondary Antibody Silver Conjugates Colorimetric detection. Permanent label
Immunohistochemistry 10nm-40nm Secondary Antibody Silver Conjugates High contrast label
Cellular Uptake  		30-80nm Standard (citrate-coated) Non-specific cellular uptake
Darkfield Microscopy 50nm-100nm Silver Conjugates
Lateral Flow/Dip-Stick Assays 20nm-80nm Standard (citrate-coated) Ideal for generation of silver conjugates through passive adsorption of antibodies to the silver nanoparticle surface.
NHS Ideal for covalent conjugation of antibodies to silver nanoparticles.
Silver Conjugates Pre-made secondary antibody conjugates
Tumor Targeting 30nm-80nm methyl (methoxy)-PEG Can in some cases be used for passive targeting of certain tumors in vivo. Inert material with low non-specific protein binding in serum.
Light Microscopy 10nm Silver Conjugates Ability to label tissue sections for both light and electron microscopy. Alternative to peroxidase and PAP-based stains. Sensitivity can be enhanced with silver enhancement techniques.
ELISA 5nm-30nm Silver Conjugates Colorimetric Detection