Protein L - 80nm Gold NanoUrchins

Protein L - 80nm Gold NanoUrchins

Gold nanoparticle core size: 80nm

Absorbance (lambda max.): 620nm

Optical density: Available at OD=3 and OD=10, select in product options

Conjugated protein: Recombinant Protein L, from P. magnus produced in E. coli

Working dilution: 1:1-1:100

Supplied in: 0.5XPBS (pH 7.4), 20% glycerol (v/v), 1% BSA

Applications

Protein L gold nanourchin conjugates are suitable for use in immunoblotting, light microscopy, and electron microscopy applications. 

Advantages of Gold Conjugates

  • Sensitive probe for detection of human and mouse antibodies containing kappa light chains.
  • Binds to a broader range of immunoglobulin classes compared to Protein A or Protein G. These include IgG, IgM, IgA, IgE and IgD.
  • Binds single-chain variable fragments (scFv) and Fab fragments that contain kappa light chains.
  • Does not bind goat or bovine IgG which makes it ideal for the specific detection of human and mouse antibodies produced in tissue culture medium containing Fetal Bovine Serum (FBS).

Storage/Stability

  • Store undiluted in storage buffer at 2-8 degree Celsius. Stable for 4 months if stored as specified.
  • Storage of conjugate at working dilution may result in performance loss.